DMPK |
Issue |
Recommendation |
Solubility |
Insoluable |
Simulated fluid replace PBS buffer |
Permeability |
Poor recovery |
Addition of BSA,FBS |
Transporter |
Substrate, or inhibitor |
Specific transporter, hMDR1 Knockin MDCKll Cells Or, hBCRP Knockin MDCKll Cells |
Bioanalysis |
Unstable |
Deactivate enzyme in plasma or blood immediately |
Bioanalysis |
Non specific binding |
Detergent |
Bioanalysis |
Fragment inLs-MS ion source |
Optimized MS condition |
Metabolism |
Diverse metabolism |
Comprehensive metabolism system: microsome, hepatocyte, S9,cytosol;AO,UGT activity; CYP phenotyping |
PPB |
Unstable |
Enzyme inhibitor |
In vivo PK |
Low bioavailability |
No fasting |